In the Pelletier lab we are fascinated with how cellular machines are built and function to regulate myriad cellular processes. Such “machines”, most commonly referred to as organelles when membrane bound (e.g. mitochondria, peroxisomes and the Golgi apparatus) or non-membrane bound (e.g. the centrosome) undergo dramatic morphological and functional changes that are tightly coordinated with the cell cycle. Our interests range from molecular mechanisms that govern centrosome-related processes in human cells including centriole duplication to pericentriolar material assembly, ciliogenesis, cell motility and mitotic spindle formation.  Our studies revolve around cutting-edge quantitative imaging approaches (from low- to super-resolution), high-content screening with emphasis on advanced morphometric measurements ranging from single cells to developing tissues/organoids. We remain keenly interested in the use of functional proteomics to systematically elucidate protein-protein interactions that are essential to maintain proper centrosome and cilia function during animal development. As such, we are especially interested in linking functional proteomics studies, genome-scale RNAi and CRISPR/Cas9 screens to understand, at the molecular level, how centrosome-cilia related processes can go awry in cancer and other clinically relevant disorders like microcephaly and myriad ciliopathies and how their function ensures normal tissue homeostasis. For more details on specific research themes click here.